Publications

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Kobayashi T, Miyashita T, Murayama T, Toyoshima YY.(2017). Dynactin has two antagonistic regulatory domains and exerts opposing effects on dynein motility. PLoS One. 12(8):e0183672
Ichikawa M, Saito K, Yanagisawa HA, Yagi T, Kamiya R, Yamaguchi S, Yajima J, Kushida Y, Nakano K, Numata O, Toyoshima YY.(2015). Axonemal dynein light chain-1 locates at the microtubule-binding domain of the heavy chain. Mol Biol Cell. 26(23):4236-47
Maheshwari A, Obbineni JM, Bui KH, Shibata K, Toyoshima YY, Ishikawa T.(2015). - and -Tubulin Lattice of the Axonemal Microtubule Doublet and Binding Proteins Revealed by Single Particle Cryo-Electron Microscopy and Tomography. Structure. 23(9):1584-95
Yamaguchi S, Saito K, Sutoh M, Nishizaka T, Toyoshima YY, Yajima J. (2015). Torque generation by axonemal outer-arm dynein. Biophys J. 108(4):872-9
Torisawa T, Ichikawa M, Furuta A, Saito K, Oiwa K, Kojima H, Toyoshima YY, Furuta K. (2014). Autoinhibition and cooperative activation mechanisms of cytoplasmic dynein. Nat Cell Biol. 16(11):1118-24
Nishikawa Y, Oyama T, Kamiya N, Kon T, Toyoshima YY, Nakamura H, Kurisu G. (2014). Structure of entire stalk region of Dynein motor domain. J Mol Biol. 426(19):3232-45
Obinata T, Amemiya S, Takai R, Ichikawa M, Toyoshima YY, Sato N. (2014). Sea Lily Muscle Lacks a Troponin-Regulatory System, While it Contains Paramyosin. Zoological Science 31(3):122-8
Furuta K, Furuta A, Toyoshima YY, Amino M, Oiwa K, Kojima H. (2013). Measuring collective transport by defined numbers of processive and nonprocessive kinesin motors. Proc Natl Acad Sci U S A. 110(2):501-6
Shibata K, Miura M, Watanabe Y, Saito K, Nishimura A, Furuta K, Toyoshima YY. (2012). A single protofilament is sufficient to support unidirectional walking of dynein and kinesin. PLoS One 7(8):e42990
Shahpasand K, Uemura I, Saito T, Asano T, Hata K, Shibata K, Toyoshima Y, Hasegawa M, Hisanaga S. (2012). Regulation of mitochondrial transport and inter-microtubule spacing by tau phosphorylation at the sites hyperphosphorylated in Alzheimer's disease. J Neurosci. 32(7):2430-41
Kitai T, Watanabe Y, Toyoshima, YY, Kobayashi T, Murayama T, Sakaue H, Suzuki H, Takahagi T. (2011). Simple Method of Synthesizing Nickel--Nitrilotriacetic Acid Gold Nanoparticles with a Narrow Size Distribution for Protein Labeling. JJpn. J. Appl. Phys. 50(9):095002-095002-4
Ichikawa M, Watanabe Y, Murayama T, Toyoshima YY. (2011). Recombinant human cytoplasmic dynein heavy chain 1 and 2: observation of dynein-2 motor activity in vitro. FEBS Lett. 585(15):2419-23
Torisawa T, Nakayama A, Furuta K, Yamada M, Hirotsune S, Toyoshima YY. (2011). Functional dissection of LIS1 and NDEL1 towards understanding the molecular mechanisms of cytoplasmic dynein regulation. J Biol Chem. 286(3):1959-65
Miura M, Matsubara A, Kobayashi T, Edamatsu M, Toyoshima YY. (2010). Nucleotide-dependent behavior of single molecules of cytoplasmic dynein on microtubules in vitro. FEBS Lett. 584(11):2351-2355 Furuta K, Edamatsu M, Maeda Y, Toyoshima YY. (2008). Diffusion and directed movement: In vitro motile properties of fission yeast kinesin-14 Pkl1. J Biol Chem. 283(52):36465-36473
Yamada M, Toba S, Yoshida Y, Haratani K, Mori D, Yano Y, Mimori-Kiyosue Y, Nakamura T, Itoh K, Fushiki S, Setou M, Wynshaw-Boris A, Torisawa T, Toyoshima YY, Hirotsune S. (2008). LIS1 and NDEL1 coordinate the plus-end-directed transport of cytoplasmic dynein. EMBO J. 27(19): 2471-2483
Taba T, Edamatsu M, Toba S, Shibata K, Imafuku Y, Toyoshima YY, Tawada K, Yamada A. (2008).Direction and speed of microtubule movements driven by kinesin motors arranged on catchin thick filaments. Cell Motil Cytoskeleton. 65(10): 816-826.
Shimizu Y, Kato Y, Morii H, Edamatsu M, Toyoshima YY, Tanokura M. (2008). The dynein stalk head, the microtubule binding-domain of dynein: NMR assignment and ligand binding. J Biomol NMR. 41(2): 89-96.
Furuta K, Toyoshima YY. (2008). Minus-End-Directed Motor Ncd Exhibits Processive Movement that Is Enhanced by Microtubule Bundling In Vitro. Curr Biol. 18(2): 152-157.

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Mori D, Yano Y, Toyo-oka K, Yoshida N, Yamada M, Muramatsu M, Zhang D, Saya H, Toyoshima YY, Kinoshita K, Wynshaw-Boris A, Hirotsune S. (2007). NDEL1 phosphorylation by Aurora-A kinase is essential for centrosomal maturation, separation, and TACC3 recruitment. Mol Cell Biol. 27(1): 352-367.
Toba, S., Watanabe, T. M., Yamaguchi-Okimoto, L., Toyoshima, Y. Y., Higuchi, H. (2006). Overlapping hand-over-hand mechanism of single molecular motility of cytoplasmic dynein. Proc Natl Acad Sci USA 103(15): 5741-5.
Kobayashi, T., Shiroguchi, K., Edamatsu, M., Toyoshima, Y. Y. (2006). Microtubule-binding properties of dynactin p150 expedient for dynein motility. Biochem Biophys Res Commun 340(1): 23-8.
Toyoshima, Y. Y. (2006). wזEiƐڒx LlV-Ǒݍp̑lȃptH[}X `j_yf Tanpakushitsu Kakusan Koso. May;51(No.6):573-8.
Toyo-Oka K, Sasaki S, Yano Y, Mori D, Kobayashi T, Toyoshima YY, Tokuoka SM, Ishii S, Shimizu T, Muramatsu M, Hiraiwa N, Yoshiki A, Wynshaw-Boris A, Hirotsune S. (2005). Recruitment of katanin p60 by phosphorylated NDEL1, an LIS1 interacting protein, is essential for mitotic cell division and neuronal migration. Hum Mol Genet. 14(21): 3113-3128.
Morii H, Shimizu T, Mizuno N, Edamatsu M, Ogawa K, Shimizu Y, Toyoshima YY. (2005). Removal of tightly bound ADP induces distinct structural changes of the two tryptophan-containing regions of the ncd motor domain.J Biochem. 138(1): 95-104.
Takahashi, Y., M. Edamatsu and Y. Y. Toyoshima (2004). Multiple ATP-hydrolyzing sites that potentially function in cytoplasmic dynein. Proc Natl Acad Sci USA 101(35): 12865-9.
Kon, T., Nishiura, M., Ohkura, R., Toyoshima, Y. Y., Sutoh, K. (2004) Distinct functions of nucleotide-binding/hydrolysis sites in the four AAA modules of cytoplasmic dynein.Biochemistry 43, 11266-11274.
Toba, S. and Y. Y. Toyoshima (2004). Dissociation of double-headed cytoplasmic dynein into single-headed species and its motile properties.Cell Motil Cytoskeleton 58(4): 281-9.
Mizuno, N., S. Toba, M. Edamatsu, J. Watai-Nishii, N. Hirokawa, Y. Y. Toyoshima and M. Kikkawa (2004). Dynein and kinesin share an overlapping microtubule-binding site. Embo J 23(13): 2459-67.
Toyoshima, Y. Y. (2004) wזEɂ`̈ꐶx זEAƃ[^[` `j_yf Tanpakushitsu Kakusan Koso. May;49(7 Suppl):935-7.
Nishiura, M., Kon, T., Shiroguchi, K., Ohkura, R., Shima, T., Toyoshima, Y.Y. & Sutoh, K. (2004) A single-headed recombinant fragment of Dictyostelium cytoplasmic dynein can drive the robust sliding of microtubules.J. Biol.Chem. 279, 2799-22802.
Toba, S., T. M. Gibson, K. Shiroguchi, Y. Y. Toyoshima and D. J. Asai (2004). Properties of the full-length heavy chains of Tetrahymena ciliary outer arm dynein separated by urea treatment. Cell Motil Cytoskeleton 58(1): 30-8.
@ Ando, T., Kodera, N., Naito, Y., Kinoshita,T., Furuta, K. & Toyoshima, Y. Y. (2003) A high-speed atomic force microscope for studying biological macromolecules in action. Chem. Phys. Chem. 4. 1196-1202.
Ohsuigi, M., Tokai-Nishizumi, N., Siroguchi, K., Toyoshima, Y.Y., Inoue, JI, & Yamamoto, T. (2003) Cdc2-mediated phosphorylation of Kid controls its distribution to spindle and chromosomes.EMBO J. 22, 2091-2103.
Shiroguchi, K., M. Ohsugi, M. Edamatsu, T. Yamamoto and Y. Y. Toyoshima (2003). The second microtubule-binding site of monomeric kid enhances the microtubule affinity. J Biol Chem 278(25): 22460-5.
Nakazawa, J., Yajima, J., Usui, T., Ueki, M., Takatsuki, A., Imoto, M., Toyoshima, Y. Y., & Osada, H. (2003) A novel action of terpendole E on the motor activity of mitotic kinesin Eg5. Chemistry & Biology 10, 131-137.
Yajima, J., M. Edamatsu, J. Watai-Nishii, N. Tokai-Nishizumi, T. Yamamoto and Y. Y. Toyoshima (2003). The human chromokinesin Kid is a plus end-directed microtubule-based motor. Embo J 22(5): 1067-74.
Edamatsu, M. & Toyoshima, Y.(2003) Fission yeast synaptobrevin is involved in cytokinesis and cell elongation.
Biochem Biophys Res Commun. 301, 641-645.
Uemura, S., Kawaguchi, K., Yajima, J., Edamatsu, M., Toyoshima, Y. Y., & Ishiwata, S. (2002) Kinesin-microtubule binding is dependent on both nucleotide state and loading direction. Proc. Natl. Acad. Sci. USA 99, 5977-5981.
Yajima, J., M. C. Alonso, R. A. Cross and Y. Y. Toyoshima (2002). Direct long-term observation of kinesin processivity at low load. Curr Biol 12(4): 301-6.
Shiroguchi, K. & Toyoshima, Y. Y. (2001) Regulation of monomeric dynein activity by ATP and ADP concentrations. Cell Motility and the Cytoskeleton 49, 189-199.
Hirakawa, E., Higuchi, H., & Toyoshima, Y.Y. (2000) Processive movement of single 22S dynein molecules occurs only at low ATP concentrations. Proc. Natl. Acad. Sci. USA, 97, 2533-2537.
Toyoshima, Y. Y. (1999) LlVX[p[t@~[̍\Ƌ@\w 17(4), 439-444.
Mohri, H., Inaba, K., Kubo-Irie, M., Takai, H., & Toyoshima, Y. Y. (1999) Characterization of outer arm dynein in sea anemone, Anthopleura midori. Cell Motility and the Cytoskeleton 44, 202-208.
Toyoshima, Y.Y. (1997) wV[YEj[oCItBWbNX SD̕q[^[̎dg݁x njn[^[̑lȉ^. o 117-125.
EImafuku, Y., Toyoshima, Y.Y., & Tawada, K. (1997) Length dependence of displacement fluctuations and velocity in microtubule sliding movement driven by sea urchin sperm outer armdynein in vitro. Biophys. Chem. 67, 117-125.
Inoue, Y., Toyoshima, Y.Y., Iwane, A.H., Morimoto, S., Higuchi, H., & Yanagida, T. (1997) Movements of truncated kinesin fragments with a short or an artificial flexible neck.
Proc. Natl. Acad. Sci. USA 94, 7275-7280.
Edamatsu, M., & Toyoshima, Y.Y. (1996) Isolation and characterization of pos mutants defective in correct positioning of septum in Scizosaccharomyces pombe. Zool. Sci. 13, 235-239.
Imafuku, Y., Toyoshima, Y.Y., & Tawada, K. (1996) Fluctuation in the microtubule sliding movement driven by kinesin in vitro.@Biophys. J. 70, 878-886.
Imafuku, Y., Toyoshima, Y.Y., & Tawada, K. (1995) A Monte Carlo study for a fluctuation analysis in the in vitro motility by@protein motors. Biopys. Chem. 59, 139-153.
Sekimoto, K., Mori, N., Tawada, K., & Toyoshima, Y. Y., (1995) Symmetry breaking instabilities of an in vitro biological system. Phys.Rev.Lett. 75, 172-175.
Shimizu, T., Toyoshima, Y.Y., Edamastu, M., & Vale, R.D. (1995) Comparison of the motile and enzymatic properties of two microtubule minus-end motors, ncd and cytoplasmic dynein.
Biochemistry 34, 1575-1582.
Itakura, S., Yamakawa,H., Toyoshima, Y.Y., Ishijima A., Kojima,T., Harada, Y., Yanagida, T., Wakabayashi,T., & Sutoh, K. (1993) Force-generating domain of myosin motor. Biochem. Biophys. Res. Commun. 196, 1504-1510.
Toyoshima, Y.Y. (1993) How are myosin fragments bound to nitrocellulose film ? In "Mechanism of myofilament sliding in muscle contraction" (ed. Sugi, H. & Pollack, G.H., Plenum Publishing Co.) pp.259-265.
Shimizu, T., Toyoshima, Y.Y., & Vale, R.D. (1993) The use of ATP analogs in the study of cell motility. In "Mehods in Cell Biology" (ed. Scholey, J. Academic Press) 39, 167-177.
Johara, M., Toyoshima, Y.Y., Ishijima, A., Kojima, H., Yanagida, T., Sutoh, K. (1993) Charge-reversion mutagenesis of Dictyostelium actin to map the surface recognized by myosin during ATP-driven sliding motion. Proc. Natl. Acad. Sci. USA. 90, 2127-2131.
ESutoh, K., Ando, M., Sutoh, K., & Toyoshima, Y.Y. (1991) Site-directed mutation in actin genes of Dictyostelium discoideum. Proc. Natl. Acad. Sci. USA. 88, 7711-7714.
Toyoshima,Y.Y. (1991) Bidirectional movement of actin filaments along tracks of heavy meromyosin and native thick filaments. J. Cell Sci. suppl. 14. 83-85.
Shimizu, T., Furusawa, K., Ohashi, S., Toyoshima, Y.Y., Okuno, M., Malik, F. & Vale R.D. (1991) Nucleotide specificity of the enzymatic and motile activities of dynein, kinesin and heavy meromyosin. J. Cell Biol. 112, 1189-1197.
Toyoshima, Y.Y. (1991) Flexibility in actin-myosin motility system revealed by in vitro motility assay. Adv. Biophys. 27, 213-220.
Schwyter, D.H., Kron, S.J., Toyoshima, Y.Y., Spudich, J.A. & Reisler, E. (1990) Subtilisin cleavage of actin inhibits in vitro sliding movement of actin filaments over myosin. J. Cell Biol. 111, 465-470.
Toyoshima, Y.Y., Kron, S.J. & Spudich, J.A. (1990) The myosin step size: Measurement of the unit displacement per ATP hydrolysed in an in vitro assay. Proc. Natl. Acad. Sci. USA. 87, 7130-7134.
Kron, S.J., Toyoshima, Y.Y., Uyeda, T.Q.P. & Spudich, J.A. (1990) Assays for actin sliding movement over myosin coated surfaces. Methods Enzymol. 196, 399-416.
Toyoshima, Y.Y., Toyoshima, C. & Spudich, J.A. (1989) Bidirectional movement of actin filaments along tracks of myosin heads. Nature 341, 154-156.
Vale, R.D. & Toyoshima, Y.Y. (1989) Microtubule translocation properties of intact and proteolytically digested dyneins from Tetrahymena cilia. J. Cell Biol. 108 2327-2334.
Vale, R.D. & Toyoshima, Y.Y. (1989) In vitiro motility assays for kinesin and dynein. In "Cell Movement" (Warner, F.D. & McIntosh, R., eds.) pp.287-294, Alan R. Liss, Inc.
Vale, R.D. & Toyoshima, Y.Y. (1988) Rotation and translocation of microtubules in vitro induced by dyneins from Tetrahymena cilia. Cell 52, 459-469.
Toyoshima, Y.Y., Kron,S.J., McNally, E.M., Niebling, K.R., Toyoshima, C. and Spudich, J.A. (1987) Myosin subfragment1 is sufficient to move actin in vitro. Nature 328, 536-539.
Toyoshima, Y.Y. (1987) Chymotryptic digestion of Tetrahymena 22S dynein. II. Pathway of the degradation of 22S dynein heavy chains by chymotryptic digestion. J. Cell Biol. 105, 897-901.
Toyoshima, Y.Y. (1987) Chymotryptic digestion of Tetrahymena 22S dynein. I. Decomposition of three-headed 22S dynein to one- and two-headed particles. J. Cell Biol. 105, 887-895.
Toyoshima, Y.Y. (1985) Subunit structure of dynein,
In Proc. Yamada. Int. Cong. on Energy Transduction, Yamada Science Foundation, pp. 216-217.
Yano-Toyoshima, Y. (1985) Two heavy chains of 21S dynein from sea urchin sperm flagella. J. Biochem. 98, 767-779.
Yano-Toyoshima, Y. (1985) Subunit structure of Tetrahymena 30S dynein. Cell Motil. 5, 148-149.
Yano, Y., Mohri, H., Toyoshima, C. & Wakabayashi, T. (1983)
Molecular structure of dynein arms. J. Submicroscopic Cytol. 15, 211-215.
Yano, Y., Mohri, H., Toyoshima, C. & Wakabayashi, T. (1982)
Molecular composition and strucutre of dynein arms. In "Biological Functions of Microtubules and Related Structures" (Sakai, H., Mohri, H. & Borisy, G.G., eds.) pp. 125-135, Academic Press.
Mohri, H. & Yano, Y. (1982) Reactivation and microtubule sliding in rodent spermatozoa. Cell Motility 1, 143-147.
Yano, Y & Miki-Noumura, T. (1981) Recovery of sliding ability in arm-depleted flagellar axonemes after recombination with extracted dynein 1. J. Cell Sci. 48, 223-239.
Yano, Y & Miki-Noumura, T. (1981) Two-headed dynein arms. Biomed. Res. 2, 73-78.
Mohri, H. & Yano, Y. (1980) Analysis of mechanism of flagellar movement with golden hamster spermatozoa. Biomed. Res. 1, 552-555.
Yano, Y. & Miki-Noumura, T. (1980) Sliding velocity between outer doublet microtubules of sea-urchin sperm axonemes. J. Cell Sci. 44, 169-186.
Hata, H., Yano, Y., Mohri, T., Mohri, H. & Miki-Noumura, T. (1980) ATP-driven tubule extrusion from axonemes without outer dynein arms of sea-urchin sperm flagella. J. Cell Sci. 41, 331-340.
Hata, H., Yano, Y. & Miki-Noumura, T. (1979) ATP concentration dependency of the tubule-extrusion velocity from the axonemes. Expl. Cell Res. 122, 416-419.

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